What method did you
use to identify and detect Keratin 19 positive stem cells
in the burns wound?
Keratin
19 stem cells are primary skin stem cells. It is usually considered
that the positive expression of K-19 can only be detected in the skin
tissue during the period of embryonic development.
However, our study has found that the expression of K-19 skin stem
cells can be found in the wound of patients in the process of skin
regenerative therapy.
After the deep burns wound was treated with MEBT/MEBO for 24h, 4d, 7d,
14d, 21d and 28 d respectively, incision biopsy in the wound for
ultra-low- temperature fixation and immunofluorescence treatment, and
K-19 positive cells were identified with high multiple
electronmicroscope.
This type of cells appeared in 24th hour, reached its peak in 14th day
after MEBT/MEBO treatment, and then disappeared gradually with the
wound recuperation after 21-28 days.
The positive expression of K-19 cells is the mark of the
“embryonic” physiological healing of all layers of the
skin, and reveals the main mechanism of skin tissue organ regeneration
technique.
※ See papers listed as supporting literatures in reference [7-10].
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